Allergen Specific IgE Measurement with Polycheck Allergy: Comparison of Three Multiple Allergen Simultaneous Tests / 대한진단검사의학회지
The Korean Journal of Laboratory Medicine
;
: 465-472, 2009.
Article
in Korean
| WPRIM
| ID: wpr-170197
ABSTRACT
BACKGROUND:
The in vivo skin prick test (SPT) or in vitro detection of allergen specific IgE in serum is commonly used for the diagnosis of allergic disease. In this study, we evaluated the usefulness of a new multiple allergen simultaneous test (MAST) immunoblot assay, Polycheck Allergy (Biocheck GmbH, Germany).METHODS:
A total of 100 patients with clinical findings of allergic diseases were tested by SPT and three different MAST assays Polycheck Allergy (Biocheck GmbH, Germany), MAST CLA allergy system (Hitachi Chemical Diagnostics, USA) and Allergy Screen (R-biopharm, Germany). The results of MAST assays were compared with those of SPT.RESULTS:
Concordance rates of MAST assays with SPT were 79-100% for Polycheck Allergy, 88.9-100% for MAST CLA and 72.7-98.3% for Allergy Screen. In ROC curve analysis, significant differences were observed in four of 25 allergens analysed Alternaria, Birch, Hazelnut and D. farinae. For Alternaria and Birch, Polycheck Allergy (P0.05).CONCLUSIONS:
Since Polycheck Allergy showed similar or superior result to the others, it can be used for the detection of allergen specific IgE antibodies.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Reagent Kits, Diagnostic
/
Immunoglobulin E
/
Skin Tests
/
Allergens
/
Immunoblotting
/
ROC Curve
/
Sensitivity and Specificity
/
Area Under Curve
/
Hypersensitivity, Immediate
Type of study:
Diagnostic study
/
Prognostic study
Limits:
Adolescent
/
Adult
/
Child
/
Child, preschool
/
Female
/
Humans
/
Male
Language:
Korean
Journal:
The Korean Journal of Laboratory Medicine
Year:
2009
Type:
Article
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