Detection of Isoniazid and Rifampicin Resistance by Sequencing of katG, inhA, and rpoB Genes in Korea / 대한진단검사의학회지
The Korean Journal of Laboratory Medicine
; : 455-460, 2009.
Article
in En
| WPRIM
| ID: wpr-170199
Responsible library:
WPRO
ABSTRACT
BACKGROUND: In Korea, tuberculosis is resistant to isoniazid (INH) and/or rifampicin (RIF) in more than 10% of cases. To prevent the spread of resistant Mycobacterium tuberculosis strains, it is crucial to develop more rapid resistance detection methods. METHODS: To determine the feasibility of using direct sequencing for detecting INH- and RIF-resistant strains, the katG gene, the regulatory region of the inhA gene, and the 81-bp hot-spot region of the rpoB gene from 95 culture isolates and 46 respiratory specimens were sequenced. Total 141 culture isolates were classified by conventional drug susceptibility testing (DST) as INH(R)/RIF(R) (N=30), INH(R)/RIF(S) (N=23), INH(S)/RIF(R) (N=15), and INH(S)/RIF(S) (N=73). RESULTS: Compared with phenotypic DST, the overall sensitivity and specificity of sequencing were 83.0% (44/53) and 96.6% (85/88), respectively, for INH resistance, and 93.3% (42/45) and 100% (96/96), respectively, for RIF resistance. The rates were similar between culture isolates and respiratory specimens. Interestingly, three specimens with inhA -15C>T mutation were susceptible to INH by conventional DST. CONCLUSIONS: Detection of mutations in the katG codon 315, the inhA regulatory region, and the hot-spot region of rpoB would be useful for rapid detection of INH and RIF resistance in Korea.
Key words
Full text:
1
Index:
WPRIM
Main subject:
Oxidoreductases
/
Rifampin
/
Bacterial Proteins
/
Catalase
/
Sensitivity and Specificity
/
Sequence Analysis, DNA
/
Drug Resistance, Multiple, Bacterial
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Republic of Korea
/
Genotype
/
Isoniazid
Type of study:
Diagnostic_studies
Limits:
Humans
Country/Region as subject:
Asia
Language:
En
Journal:
The Korean Journal of Laboratory Medicine
Year:
2009
Type:
Article