Improvement of the Inferior Epigastric Artery Flap Viability Using Adenovirus-mediated VEGF and COMP-angiopoietin-1
Journal of the Korean Society of Plastic and Reconstructive Surgeons
;
: 1-10, 2009.
Article
in Korean
| WPRIM
| ID: wpr-170256
ABSTRACT
PURPOSE:
Partial necrosis of skin flaps remains a substantial problem in reconstructive surgery. We investigated the potential use of an adenovirus vector encoding the VEGF, COMP-angiopoietin-1 gene in an attempt to promote the viability of the inferior epigastric artery flap in a rat model.METHODS:
Three by six cm lower abdominal transverse skin flaps, supplied only by the left inferior epigastric artery, were designed. After skin flap elevation, the adenovirus VEGF and adenovirus COMP-angiopoietin-1 were injected into the distal portion of the flap, which has a high tendency of developing flap ischemia. Control animals were injected with the same volume of normal saline. On 3, 7 and 14 days after the flap elevation, the flap survival and vascularization were assessed using Visitrak digital(R), CD31 immunohistochemistry in addition to evaluating the general histological characteristics.RESULTS:
There was a significant increase in the mean percentage of flap viability by 89.8%, 91.1% and 94.8% in flaps transfected with adenovirus VEGF, COMP- angiopoietin-1, coadministraion of VEGF and COMP- angiopoietin-1 at seven days, and by 95.6%, 94.8% and 96.3% at 14 days. Histological assessment revealed that there were more blood vessels formed after adenovirus with VEGF, COMP-angiopoietin-1 or VEGF plus COMP- angiopoietin-1 than with adenovirus Lac Z.CONCLUSION:
The results of this study suggest that adenovirus-mediated VEGF, COMP-angiopoietin-1 gene therapy, promote therapeutic angiogenesis in patients that undergo reconstructive procedures. Key Words
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Skin
/
Blood Vessels
/
Immunohistochemistry
/
Genetic Therapy
/
Adenoviridae
/
Epigastric Arteries
/
Angiopoietin-1
/
Vascular Endothelial Growth Factor A
/
Ischemia
/
Necrosis
Type of study:
Prognostic study
Limits:
Animals
/
Humans
Language:
Korean
Journal:
Journal of the Korean Society of Plastic and Reconstructive Surgeons
Year:
2009
Type:
Article
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