In vitro characterization of human dental pulp stem cells isolated by three different methods
Restorative Dentistry & Endodontics
;
: 283-295, 2016.
Article
in English
| WPRIM
| ID: wpr-170668
ABSTRACT
OBJECTIVES:
In this study, we characterized human dental pulp cells (HDPCs) obtained by different culture methods to establish the most suitable methodology for dental tissue engineering and regenerative endodontic applications. MATERIALS ANDMETHODS:
HDPCs were isolated by the outgrowth method (HDPCs-OG), the enzymatic digestion method (collagenase/dispase/trypsin, HDPCs-ED), or the combination of both methods (HDPCs-Combined). The expression of mesenchymal stem cell markers (CD105, CD90, and CD73) was investigated. In vitro differentiation capacities of HDPCs into adipogenic, osteogenic, and chondrogenic lineages were compared. Differentiation markers were analyzed by quantitative reverse-transcription polymerase chain reaction (RT-PCR) and western blotting.RESULTS:
Our data indicated that whole HDPCs-ED, HPDCs-OG, and HDPCs-Combined could be differentiated into adipogenic, chrondrogenic, and osteogenic cell types. However, we found that the methods for isolating and culturing HDPCs influence the differentiation capacities of cells. HDPCs-OG and HDPCs-ED were preferably differentiated into adipogenic and osteogenic cells, respectively. Differentiation markers shown by RT-PCR and western blotting analysis were mostly upregulated in the treated groups compared with the control groups.CONCLUSIONS:
Our findings confirmed that cell populations formed by two different culture methods and the combined culture method exhibited different properties. The results of this study could provide an insight into regenerative endodontic treatment using HDPCs.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Stem Cells
/
In Vitro Techniques
/
Antigens, Differentiation
/
Blotting, Western
/
Polymerase Chain Reaction
/
Tissue Engineering
/
Dental Pulp
/
Digestion
/
Mesenchymal Stem Cells
/
Methods
Limits:
Humans
Language:
English
Journal:
Restorative Dentistry & Endodontics
Year:
2016
Type:
Article
Similar
MEDLINE
...
LILACS
LIS