Effect of Anti-Siglec-F Antibody and Reactive Oxygen Species Blocking on Histamine Release in Urinary Bladder of Ovalbumin-Treated Mice / 대한배뇨장애요실금학회지
International Neurourology Journal
;
: 122-125, 2012.
Article
in English
| WPRIM
| ID: wpr-170974
ABSTRACT
PURPOSE:
Sialic acid-binding Ig-like lectin (Siglec) is an immune inhibitory receptor that plays a role in the negative regulation of the activation of immune cells. This study aimed to evaluate the effects of anti-Siglec-F on plasma and urinary histamine levels in ovalbumin (OVA)-challenged urinary bladder in mice.METHODS:
Thirty BALB/c mice were used. In group I (control group, n=5), mice were sensitized with OVA and challenged with saline. In group II (OVA challenge group, n=5), OVA was used for intraperitoneal sensitization and intravesical challenge. The challenged mice in group III (control immunoglobulin G [IgG] group, n=5) and those in group IV (anti-Siglec-F group, n=5) were intraperitoneally pretreated with rabbit control IgG or anti-Siglec-F antibody, respectively. In groups V (N-acetylcysteine [NAC] in OVA challenge group, n=5) and VI (control NAC only, n=5), mice were pretreated with NAC.RESULTS:
Urinary histamine concentrations were significantly higher 7 days after intravesical OVA challenge (P<0.01), whereas plasma histamine levels were not. Pretreatment with anti-Siglec-F antibody significantly prevented the increase in urinary histamine release (P<0.05), whereas pretreatment with the IgG antibody control did not. Also, pretreatment of the OVA challenge group with NAC did not affect the histamine concentration in either urine or plasma.CONCLUSIONS:
Systemic anti-Siglec-F treatment showed anti-allergic effects at least on local histamine release, particularly in the lower urinary bladder.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Ovum
/
Plasma
/
Urinary Bladder
/
Immunoglobulin G
/
Histamine
/
Ovalbumin
/
Reactive Oxygen Species
/
Sialic Acid Binding Immunoglobulin-like Lectins
/
Histamine Release
Limits:
Animals
Language:
English
Journal:
International Neurourology Journal
Year:
2012
Type:
Article
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