Adenovirus Expressing Human Interferon Inhibits Replication of Foot and Mouth Disease Virus and Reduces Fatal Rate in Mice
Journal of Bacteriology and Virology
;
: 224-231, 2012.
Article
in English
| WPRIM
| ID: wpr-170983
ABSTRACT
Interferon is an important cytokine that plays a critical role in the initial host defense against viral infection. Recombinant human adenoviruses expressing human interferon-alpha (Ad-HIFNalpha) or pig interferon-beta fused with interleukin-18 (Ad-PIFNbeta-IL18) were constructed and used to induce an early protective response against foot and mouth disease (FMD). To analyze the antiviral effect, bovine thyroid and porcine kidney IBRS-2 cells and ICR mice were treated with Ad-HIFNalpha, Ad-PIFNbeta-IL18, and cocktail of Ad-HIFNalpha and Ad-PIFNbeta-IL18. The survival rate of suckling mice was monitored after foot and mouth disease virus (FMDV) challenge following intra-peritoneal (IP) administration of appropriate adenovirus. Indirect antigen ELISA was performed to evaluate inhibition of FMDV replication following challenge with the FMDV O, A, or Asia 1 serotypes in vitro. These recombinant adenoviruses reduced the replication of FMDV in susceptible cells, thereby decreasing the fatality in mice, suggesting that they can be a useful control method for the early protection against FMD infection in livestock after field trial.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Asia
/
Thyroid Gland
/
Enzyme-Linked Immunosorbent Assay
/
Adenoviridae
/
Adenoviruses, Human
/
Survival Rate
/
Interferons
/
Interferon-beta
/
Interferon-alpha
/
Interleukin-18
Limits:
Animals
/
Humans
Country/Region as subject:
Asia
Language:
English
Journal:
Journal of Bacteriology and Virology
Year:
2012
Type:
Article
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