Grape extracts suppress the formation of preneoplastic foci and activity of fatty acid synthase in rat liver
Experimental & Molecular Medicine
;
: 371-378, 2003.
Article
in English
| WPRIM
| ID: wpr-171364
ABSTRACT
This study was conducted to examine the effects of dietary grape extracts on preneoplastic foci formation in rat hepatocarcinogenesis, and related hepatic enzymes. Male Sprague-Dawley rats were fed basal diet or grape diet containing 15% concentrated grape extracts (68 bricks). The grape diet groups were divided into whole-period grape diet group (DEN-GW; grape diet group fed throughout experimental period) and postinitiation grape diet group (DEN-GP; grape diet group fed from post initiation stage) according to the starting time point of the grape diet. Hepatocarcinogenesis was induced by diethylnitrosamine (DEN; 200 mg/kg bw) and 2/3 partial hepatectomy (DEN-B; DEN-treated basal diet group, DEN-GW, and DEN-GP groups), while the control group treated with saline and sham operation (Control group). The formation of placental glutathione (GSH) S-transferase positive (GST-P(+)) foci in DEN-GW group was moderately but significantly suppressed, however, not in DEN- GP group. Thiobarbituric acid reactive substances content of DEN-GW group was significantly lower than that of DEN-B group. The activity of fatty acid synthase (FAS) in the grape diet groups was decreased about 1/2 of the DEN-B group. The content of GSH and GSH peroxidase activity were increased by carcinogen treatment, but not modulated by grape diet. The activities of GSH S-transferase, p-nitrophenol hydroxylase, and catalase were not affected by diet or treatment. Conclusively, the grape diet-induced reduction of FAS activity that was expressed highly in neoplastic tissues, might be one of the contributing mechanisms of hepatic cancer prevention.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Organ Size
/
Body Weight
/
Plant Extracts
/
Catalase
/
Cell Transformation, Neoplastic
/
Administration, Oral
/
Rats, Sprague-Dawley
/
Dietary Supplements
/
Vitis
/
Diet
Limits:
Animals
Language:
English
Journal:
Experimental & Molecular Medicine
Year:
2003
Type:
Article
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