Effect of glucose on the expression of c-myc gene in cultured RINm5F cell
Journal of Korean Medical Science
;
: 246-250, 1991.
Article
in English
| WPRIM
| ID: wpr-172060
ABSTRACT
The study was designed to examine the effect of glucose on the expression of c-myc gene in cultured RINm5F cells. After monolayer culture was established in RPMI 1640 media supplemented with 10% fetal calf serum (FCS), the cells were cultured in various concentrations of glucose and 1 or 10% FCS for another 24 hours. A mRNA was extracted from the cultured cells by a single step method, and Northern analysis was done to detect RNA band. A 0.5 kilobase single band was detected as c-myc mRNA. The expression of c-myc gene mRNA was reduced with increased concentration of glucose with 1% FCS. However, supplementation of 10% FCS abolished the effect of glucose on expression of c-myc gene. These findings suggested that glucose in conjunction with other growth promoting factors played an important role in expression of oncogene and cell growth in RINm5F cells.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Pancreatic Neoplasms
/
Tumor Cells, Cultured
/
Gene Expression Regulation, Neoplastic
/
Cell Line
/
Genes, myc
/
Glucose
/
Insulinoma
Limits:
Animals
Language:
English
Journal:
Journal of Korean Medical Science
Year:
1991
Type:
Article
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