Analysis of Discrepancies Between G-banding and FISH in Hematologic Abnormalities / 대한임상병리학회지
Korean Journal of Clinical Pathology
;
: 445-450, 2001.
Article
in Korean
| WPRIM
| ID: wpr-172173
ABSTRACT
BACKGROUND:
The effective treatment of hematologic malignancies depends upon application of different therapeutic strategies by selecting patients known as the high risk group and the detection of malignant cells that can not be distinguished during following-up. We compared the results of G-banding and fluorescence in situ hybridization (FISH), which are used most frequently in detecting genetic changes, with the respect to investigating the discrepancies between these methods.METHODS:
G-banding and FISH were performed on 919 consecutive specimens from 304 patients with hematologic malignancies. As for FISH, we covered most of the more frequent gene-tic changes, using 18 types of FISH probe.RESULTS:
The average discrepancy between G-banding and FISH was 8.6% with a discrepancy at initial diagnosis of 6.0% and at follow-up of 11.9%, indicating greater discrepancy at follow-up after treatment. The chromosomal changes with especially large discrepancies were TEL/AML1, BCR/ABL & del(5q) (22.4%, 18.1%, and 16.2%, respectively). According to each disease, the discrepancies in acute biphenotypic leukemia (33.3%), acute lymphoblastic leukemia (14.7%), and chronic myelogenous leukemia (9.6%) were larger than average discrepancy. CONCLUSTIONS We concluded that application of FISH is effective for detecting genetic changes in hematologic malignancies. Once genetic changes are detected, follow-up with FISH would be especially effective for making an accurate assessment of the likelihood of complete remission and recurrence.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Recurrence
/
Leukemia, Biphenotypic, Acute
/
Leukemia, Myelogenous, Chronic, BCR-ABL Positive
/
Follow-Up Studies
/
In Situ Hybridization
/
Hematologic Neoplasms
/
Diagnosis
/
Precursor Cell Lymphoblastic Leukemia-Lymphoma
/
Fluorescence
Type of study:
Diagnostic study
/
Observational study
/
Prognostic study
Limits:
Humans
Language:
Korean
Journal:
Korean Journal of Clinical Pathology
Year:
2001
Type:
Article
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