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Studies of Changes of Ca2+-channels Distribution in the Activated Mouse Ova / 대한불임학회지
Korean Journal of Fertility and Sterility ; : 13-24, 2001.
Article in Korean | WPRIM | ID: wpr-173166
ABSTRACT

OBJECTIVE:

In muscle and neuronal cells, calcium channels have been classified by electrophysiological and pharmacological properties into (1) voltage-dependent Ca2+-channel(1) P/Q-type Ca2+-channel (2) N-type Ca2+-channel R(3) L-type Ca2+-channel (4) T-type Ca2+-channel (5)R-type Ca2+-channel. The present study was done in order to investigate whether there is any difference in Ca2+-channel distribution between activated and normally fertilized embryos.

METHODS:

The immunocytochemical method was used to identify the existence of voltage-dependent Ca2+-channels in parthenogenetically activated 2-cell embryos by ethanol and SrCl2 treatment. These 2-cell embryos were obtained by exposure to 6% ethanol for 6 min and to 10 mM SrCl2 for 2h.

RESULTS:

P/Q-type Ca2+ channels and L-type Ca2+-channels have been identified. Whereas, three type of Ca2+-channel P/Q-type, N-type, L-type have been identified in 2-cell embryos fertilized in vivo.

CONCLUSION:

Activation by ethanol was faster than those by SrCl2. However, there was difference in DAB staining of the embryos between ethanol and SrCl2 treatment (87.7% and 54.1%). Intensity of staining was also different between ethanol- and SrCl2-treated group. However, it has not been known why there was some difference in DAB staining and staining intensity in the present study.
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Full text: Available Index: WPRIM (Western Pacific) Limits: Animals Language: Korean Journal: Korean Journal of Fertility and Sterility Year: 2001 Type: Article

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Full text: Available Index: WPRIM (Western Pacific) Limits: Animals Language: Korean Journal: Korean Journal of Fertility and Sterility Year: 2001 Type: Article