APC Mutation Spectra in Sporadic Colon Cancer Tissue among Korean
Journal of the Korean Surgical Society
;
: 159-167, 2009.
Article
in Korean
| WPRIM
| ID: wpr-173194
ABSTRACT
PURPOSE:
APC (adenomatous polyposis coli) gene is one of the tumor-suppressor genes that acts in the early stages of cancer. Among general colon cancer patients, normal APC gene expression is deficient in 80%. It seems that APC is the most important gene in the development of colon cancer. This study was performed to analyze the mutation spectra of APC gene in sporadic colon cancer tissue from Korean patients with colon cancer.METHODS:
A total of 38 patients with sporadic colon cancer were enrolled. Colon cancer tissues were analyzed for the determination of APC gene mutation spectra by multiplex ligation-dependent probe amplification (MLPA) method using SALSA MLPA P403 APC kit (MRC-Holland, Amsterdam, NL).RESULTS:
APC gene mutations showing deletion/duplication in one or more exons were detected in 23 (60.5%) patients. Duplication in 13 patients (56.5%), duplication and deletion in 7 patients (30.4%), and deletion in 3 patients (13.1%) was detected. The incidence of APC gene mutation found in this study was highest in exon 3. From this study, no significant differences were observed with respect to clinicopathologic findings and the presence or absence of APC mutations.CONCLUSION:
The frequency of APC gene mutation was about 61% in Korean patients with colon cancer, it showed concordance with the previous reports on the frequency of APC gene mutation from Caucasian patients with sporadic colon cancer. However, in contrast to these reports, the frequency of duplication disclosed much higher than those of western countries.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Exons
/
Incidence
/
Genes, APC
/
Colon
/
Colonic Neoplasms
/
Multiplex Polymerase Chain Reaction
Type of study:
Incidence study
/
Prognostic study
Limits:
Humans
Language:
Korean
Journal:
Journal of the Korean Surgical Society
Year:
2009
Type:
Article
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