Interleukin-1beta stimulates matrix metalloproteinase-2 expression via a prostaglandin E2-dependent mechanism in human chondrocytes
Experimental & Molecular Medicine
;
: 226-232, 2004.
Article
in English
| WPRIM
| ID: wpr-173481
ABSTRACT
IL-1beta is known promote cyclooxygenase-2 (COX- 2) and matrix metalloproteinase-2 (MMP-2) expression. This study focuses on the characterization of the signaling cascade associated with IL-1beta-induced matrix metalloproteinase-2 (MMP- 2) regulation in human chondrocytes. The decrease in collagen levels in the conditioned media was prevented by a broad spectrum MMP inhibitor, suggesting that IL-1beta promotes the proteolytic process leading to MMP-2 activation. IL-1beta-related MMP-2 expression was found to be dependent on prostaglandin E2 (PGE2) production. In addition, the induction of COX-2 and MMP-2 was inhibited by the pretreatment of chondrocytes with a SB203580 or Ro 31-8220, indicating the involvement of protein kinase C (PKC) or p38 mitogen-activated protein kinase (MAPK). However, there is no cross-talk between PKC and p38 MAPK in the IL-1beta-induced MMP-2 activation. Taken together, these results demonstrated that IL-1beta induces MMP-2 expression through the PGE2-dependent mechanism in human chondrocytes.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Phosphorylation
/
Sulfonamides
/
Protein Kinase C
/
Dinoprostone
/
Up-Regulation
/
Interleukin-1
/
Prostaglandin-Endoperoxide Synthases
/
Chondrocytes
/
Matrix Metalloproteinase 2
/
P38 Mitogen-Activated Protein Kinases
Limits:
Humans
Language:
English
Journal:
Experimental & Molecular Medicine
Year:
2004
Type:
Article
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