Akt is involved in the inhibition of cell proliferation by EGF
Experimental & Molecular Medicine
;
: 491-498, 2007.
Article
in English
| WPRIM
| ID: wpr-174054
ABSTRACT
Axin is a negative regulator of the Wnt/beta-catenin pathway and is involved in the regulation of axis formation and proliferation. Involvement of Axin in the regulation of other signaling pathways is poorly understood. In this study, we investigated the involvement of Akt in growth regulation by Axin in L929 fibroblasts stimulated by EGF. Akt activity was increased by EGF treatment and Ras activation, respectively. Both the EGF- and Ras-induced Akt activations were abolished by Axin induction, as revealed by both Western blot and immunocytochemical analyses. The proliferation and Akt activation induced by EGF were decreased by Axin induction, and the effects of EGF were abolished by treatment of an Akt-specific inhibitor. Therefore, Axin inhibits EGF-induced proliferation of L929 fibroblasts by blocking Akt activation.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Repressor Proteins
/
Signal Transduction
/
Cell Line
/
Cell Nucleus
/
Ras Proteins
/
Cell Proliferation
/
Epidermal Growth Factor
/
Proto-Oncogene Proteins c-akt
/
Fibroblasts
Limits:
Animals
Language:
English
Journal:
Experimental & Molecular Medicine
Year:
2007
Type:
Article
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