Cyclooxygenase-2 promotes cell proliferation, migration and invasion in U2OS human osteosarcoma cells
Experimental & Molecular Medicine
;
: 469-476, 2007.
Article
in English
| WPRIM
| ID: wpr-174057
ABSTRACT
Osteosarcoma is the most common primary bone tumor, but the pathogenesis is not well understood. While cyclooxygeanse-2 (COX-2) is known to be closely associated with tumor growth and metastasis in several kinds of human tumors, the function of COX-2 in osteosarcoma is unclear. Therefore, to investigate the function of COX-2 in osteosarcoma, we established stable cell lines overexpressing COX-2 in U2OS human osteosarcoma cells. COX-2 overexpression as well as prostaglandin E(2) treatment promoted proliferation of U2OS cells. In addition, COX-2 overexpression enhanced mobility and invasiveness of U2OS cells, which was accompanied by increases of matrix metalloproteinase-2 and -9 (MMP-2 and -9) activities. Selective COX-2 inhibitors, NS-398 and celecoxib, inhibited cell proliferation and abrogated the enhanced mobility, invasiveness and MMP activities induced by COX-2 overexpression. These results suggest that COX-2 is directly associated with cell proliferation, migration and invasion in human osteosarcoma cells, and the therapeutic value of COX-2 inhibitors should be evaluated continuously.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Pyrazoles
/
Sulfonamides
/
Bone Neoplasms
/
Dinoprostone
/
Osteosarcoma
/
Cell Movement
/
Matrix Metalloproteinase 2
/
Matrix Metalloproteinase 9
/
Cell Line, Tumor
/
Cell Proliferation
Limits:
Humans
Language:
English
Journal:
Experimental & Molecular Medicine
Year:
2007
Type:
Article
Similar
MEDLINE
...
LILACS
LIS