The Genetically Modified Polysialylated Form of Neural Cell Adhesion Molecule-Positive Cells for Potential Treatment of X-Linked Adrenoleukodystrophy
Yonsei Medical Journal
;
: 246-252, 2013.
Article
in English
| WPRIM
| ID: wpr-17422
ABSTRACT
PURPOSE:
Cell transplantation of myelin-producing exogenous cells is being extensively explored as a means of remyelinating axons in X-linked adrenoleukodystrophy. We determined whether 3,3',5-Triiodo-L-thyronine (T3) overexpresses the ABCD2 gene in the polysialylated (PSA) form of neural cell adhesion molecule (NCAM)-positive cells and promotes cell proliferation and favors oligodendrocyte lineage differentiation. MATERIALS ANDMETHODS:
PSA-NCAM+ cells from newborn Sprague-Dawley rats were grown for five days on uncoated dishes in defined medium with or without supplementation of basic fibroblast growth factor (bFGF) and/or T3. Then, PSA-NCAM+ spheres were prepared in single cells and transferred to polyornithine/fibronectin-coated glass coverslips for five days to determine the fate of the cells according to the supplementation of these molecules. T3 responsiveness of ABCD2 was analyzed using real-time quantitative polymerase chain reaction, the growth and fate of cells were determined using 5-bromo-2-deoxyuridine incorporation and immunocytochemistry, respectively.RESULTS:
Results demonstrated that T3 induces overexpression of the ABCD2 gene in PSA-NCAM+ cells, and can enhance PSA-NCAM+ cell growth in the presence of bFGF, favoring an oligodendrocyte fate.CONCLUSION:
These results may provide new insights into investigation of PSA-NCAM+ cells for therapeutic application to X-linked adrenoleukodystrophy.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Sialic Acids
/
Stem Cells
/
Thyroid Hormones
/
Triiodothyronine
/
Bromodeoxyuridine
/
Immunohistochemistry
/
Cell Differentiation
/
Fibroblast Growth Factor 2
/
Fibronectins
/
Rats, Sprague-Dawley
Limits:
Animals
Language:
English
Journal:
Yonsei Medical Journal
Year:
2013
Type:
Article
Similar
MEDLINE
...
LILACS
LIS