Mouse Model of IL-17-Dominant Rhinitis Using Polyinosinic-Polycytidylic Acid
Allergy, Asthma & Immunology Research
;
: 540-549, 2017.
Article
in English
| WPRIM
| ID: wpr-174357
ABSTRACT
Interleukin (IL)-17 plays an important role in rhinitis and the level thereof correlates with the severity of disease. However, no mouse model for IL-17-dominant rhinitis has yet been developed. Our objective was to establish a mouse model of IL-17-dominant rhinitis via intranasal application of polyinosinic-polycytidylic acid (abbreviated as Poly(IC)). Mice were divided into 6 groups (n=8 for each group); 1) 1 negative control group, 2) 1 positive control group (OVA/alum model), 3) 2 Poly(IC) groups (10 or 100 µg), and 4) 2 OVA/Poly(IC) groups (10 or 100 µg). The positive control group was treated with the conventional OVA/alum protocol. In the Poly(IC) and OVA/Poly(IC) groups, phosphate-buffered saline or an OVA solution plus Poly(IC) were administered. The OVA/Poly(IC) groups exhibited significantly greater neutrophil infiltration and increased IL-17/interferon γ expression compared with the other groups. However, the levels of total immunoglobulin E (IgE), OVA-specific IgE, eosinophil infiltration, IL-4, IL-5, IL-6, and IL-10 were significantly lower in the OVA/Poly(IC) groups than in mice subjected to conventional Th2-dominant OVA/alum treatment (the positive control group). IL-17 and neutrophil measurement, chemokine (C-X-C motif) ligand 1 immunohistochemistry, and confocal microscopy revealed increased numbers of IL-17-secreting cells in the nasal mucosa of the OVA/Poly(IC) groups, which included natural killer cells, CD4 T cells, and neutrophils. In conclusion, we developed a mouse model of IL-17-dominant rhinitis using OVA together with Poly(IC). This model will be useful in research on neutrophil- or IL-17-dominant rhinitis.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Ovum
/
Immunoglobulin E
/
Immunoglobulins
/
Immunohistochemistry
/
Killer Cells, Natural
/
T-Lymphocytes
/
Rhinitis
/
Interleukins
/
Interleukin-4
/
Interleukin-5
Type of study:
Practice guideline
/
Prognostic study
Limits:
Animals
Language:
English
Journal:
Allergy, Asthma & Immunology Research
Year:
2017
Type:
Article
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