Expression of major histocompatibility complex antigen in Lewis rat cornea
Korean Journal of Ophthalmology
; : 66-71, 1994.
Article
in En
| WPRIM
| ID: wpr-175239
Responsible library:
WPRO
ABSTRACT
Fresh rat corneas as well as corneas preserved in several different corneal preservation media were stained with Avidin-Biotin-peroxidase Complex method in order to evaluate major histocompatibility complex (MHC) antigen expression. In fresh corneas, class I antigen was identified in corneal epithelium, stroma and endothelium. Class II antigen was identified only in stroma. In corneas preserved in the media which contained chondroitin and dextran for 7 days, class I antigen was somewhat decreased but class II antigen was increased. In corneas preserved in the medium which contained insulin or epidermal growth factor for 7 days, class II antigens seemed to be increased compaired to the fresh cornea. Expression of MHC antigens of corneas in the medium with fetal bovine serum were similar to those of fresh corneas.
Key words
Full text:
1
Index:
WPRIM
Main subject:
Organ Preservation
/
Rats, Inbred Lew
/
Histocompatibility Antigens Class I
/
Histocompatibility Antigens Class II
/
Immunoenzyme Techniques
/
Cornea
/
Culture Media
/
Major Histocompatibility Complex
Type of study:
Prognostic_studies
Limits:
Animals
Language:
En
Journal:
Korean Journal of Ophthalmology
Year:
1994
Type:
Article