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MiR-17-5p modulates osteoblastic differentiation and cell proliferation by targeting SMAD7 in non-traumatic osteonecrosis
Experimental & Molecular Medicine ; : e107-2014.
Article in English | WPRIM | ID: wpr-175272
ABSTRACT
MicroRNAs (miRNAs) have recently been recognized to have a role in human orthopedic disorders. The objective of our study was to explore the expression profile and biological function of miRNA-17-5p (miR-17-5p), which is well known to be related to cancer cell proliferation and invasion, in osteoblastic differentiation and in cell proliferation. The expression levels of miR-17-5p in the femoral head mesenchymal stem cells of 20 patients with non-traumatic osteonecrosis (ON) and 10 patients with osteoarthritis (OA) were examined by quantitative reverse transcription-PCR (qRT-PCR). Furthermore, the interaction between miR-17-5p and SMAD7 was observed. We found that in non-traumatic ON samples the level of mature miR-17-5p was significantly lower than that of OA samples (P=0.0002). By targeting SMAD7, miR-17-5p promoted nuclear translocation of beta-catenin, enhanced expression of COL1A1 and finally facilitated the proliferation and differentiation of HMSC-bm cells. We also demonstrated that restoring expression of SMAD7 in HMSC-bm cells partially reversed the function of miR-17-5p. Together, our data suggested a theory that dysfunction of a network containing miR-17-5p, SMAD7 and beta-catenin could contribute to ON pathogenesis. The present study prompts the potential clinical value of miR-17-5p in non-traumatic ON.
Subject(s)
Full text: Available Index: WPRIM (Western Pacific) Main subject: Osteoarthritis / Osteoblasts / Osteogenesis / Osteonecrosis / Base Sequence / Signal Transduction / Cell Differentiation / Cell Line / Gene Expression Regulation / MicroRNAs Limits: Adult / Female / Humans / Male Language: English Journal: Experimental & Molecular Medicine Year: 2014 Type: Article

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Full text: Available Index: WPRIM (Western Pacific) Main subject: Osteoarthritis / Osteoblasts / Osteogenesis / Osteonecrosis / Base Sequence / Signal Transduction / Cell Differentiation / Cell Line / Gene Expression Regulation / MicroRNAs Limits: Adult / Female / Humans / Male Language: English Journal: Experimental & Molecular Medicine Year: 2014 Type: Article