Excess of leptin inhibits hypothalamic KiSS-1 expression in pubertal mice / 소아과
Korean Journal of Pediatrics
;
: 337-343, 2012.
Article
in English
| WPRIM
| ID: wpr-175380
ABSTRACT
PURPOSE:
Leptin has been considered a link between metabolic state and reproductive activity. Defective reproductive function can occur in leptin-deficient and leptin-excessive conditions. The aim of this study was to examine the effects of centrally injected leptin on the hypothalamic KiSS-1 system in relation to gonadotropin-releasing hormone (GnRH) action in the initial stage of puberty.METHODS:
Leptin (1 microg) was injected directly into the ventricle of pubertal female mice. The resultant gene expressions of hypothalamic GnRH and KiSS-1 and pituitary LH, 2 and 4 hours after injection, were compared with those of saline-injected control mice. The changes in the gene expressions after blocking the GnRH action were also analyzed.RESULTS:
The basal expression levels of KiSS-1, GnRH, and LH were significantly higher in the pubertal mice than in the prepubertal mice. The 1-microg leptin dose significantly decreased the mRNA expression levels of KiSS-1, GnRH, and LH in the pubertal mice. A GnRH antagonist significantly increased the KiSS-1 and GnRH mRNA expression levels, and the additional leptin injection decreased the gene expression levels compared with those in the control group.CONCLUSION:
The excess leptin might have suppressed the central reproductive axis in the pubertal mice by inhibiting the KiSS-1 expression, and this mechanism is independent of the GnRH-LH-estradiol feedback loop.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Axis, Cervical Vertebra
/
RNA, Messenger
/
Luteinizing Hormone
/
Carbonates
/
Gene Expression
/
Gonadotropin-Releasing Hormone
/
Puberty
/
Leptin
/
Aluminum Hydroxide
Limits:
Animals
/
Female
/
Humans
Language:
English
Journal:
Korean Journal of Pediatrics
Year:
2012
Type:
Article
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