The Effect of the Transcriptional Regulation of Sp1 for TGF-beta1 and CTGF Expression in Scar Formation
Journal of the Korean Society of Plastic and Reconstructive Surgeons
;
: 39-45, 2006.
Article
in Korean
| WPRIM
| ID: wpr-175993
ABSTRACT
This study is to examine the relationship between TGF-b1 expression and CTGF expression, and to evaluate the effect of Sp1 blockade on the expression of TGF-b1, CTGF and extracellular genes, clones of fibroblasts stably transfected with Sp1 decoy ODN. R-Sp1 decoy ODN was highly resistant to degradation by nucleases or serum, compared to the linear or phosphorothioated-Sp1 decoy ODN. Skin wounds were created on the back of 36 anesthetized rats. They were divided into four groups-the rats with normal skin, with wounded skin without decoy, with wounded skin injected with R-Sp1 decoy, and with wounded skin injected with mismatched R-Sp1 decoy, respectively. Skins were collected at 3rd, 5th, 7th, 14th day after wounding. Cellular RNA was extracted by RT-PCR analysis. TGF-beta1 and CTGF were deeply related with skin fibrosis during scar formation and it appeared that TGF-beta1 may cause the induction of CTGF expression. R-Sp1 decoy ODN inhibited TGF-beta1 and CTGF expression both in cultured fibroblasts and in the skin of rats. These results indicate that targeting Sp1 with R-type decoy efficiently blocks extracellular matrix gene expression, and suggest an important new therapeutic approach to control the scarring in normal wound healing and fibrotic disorders.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Skin
/
Wound Healing
/
Wounds and Injuries
/
Fibrosis
/
RNA
/
Gene Expression
/
Cicatrix
/
Clone Cells
/
Extracellular Matrix
/
Transforming Growth Factor beta1
Limits:
Animals
Language:
Korean
Journal:
Journal of the Korean Society of Plastic and Reconstructive Surgeons
Year:
2006
Type:
Article
Similar
MEDLINE
...
LILACS
LIS