Study on the application of the preparation and storage of cord blood cells in the Ho Chi Minh Center for Hematology and Blood Transfusion

ABSTRACT

We study characteristic of 306 cord blood samples collected at Hung Vuong hospital from 1997 year to 2000 year. The results were the following as compared with foreign authors’ results we revealed that the median volume of Vietnamese newborn’s cord blood was smaller, there were not significantly different on the median number of nucleated cells, the median number of CD34+ stem cells, the percentage of CD34+ cells as compared with mononuclear cells and the value of the use of cord blood for transplantation; the average percentage of immunologic makers on cord blood cells and peripheral blood cells and in our study was comparable with other author’s results but the percentage of CD2+ and CD19+; hematologic indexes of cord blood in our study were not significantly different as compared with other but the number of granulocytes, red blood cells and hematocrite were smaller; the incidence of infectious diseases was the following bacterial infection 5.9%; viral infection HbsAg(+) 1.79%; anti HCV(+) 1.34%; anti HIV(+) 0.44%; VDRL(+) 0%; anti HTLV-1.2 0; anti CMV IgM 0.90%. Anti HBc IgM 5.3%. Conclusion as compared with foreign authour results we revealed that the median volume of Vietnamses newborn’s cord blood was smaller and there were not significantly different on the median number of nucleated cells, the median number of CD34+ stem cells, the percentage of CD34+ cells as compared with mononuclear cells and the average percentage of immunologic makers. So it is useful in transplantation to treat malignant and congenital diseases in Vietnam.