Altered expression of norepinephrine transporter and norepinephrine in human placenta cause pre-eclampsia through regulated trophoblast invasion / 대한생식의학회지
Clinical and Experimental Reproductive Medicine
;
: 12-22, 2013.
Article
in English
| WPRIM
| ID: wpr-176446
ABSTRACT
OBJECTIVE:
We investigated the norepinephrine transporter (NET) expression in normal and pre-eclamptic placentas and analyzed the invasion activity of trophoblastic cells based on norepinephrine (NE)-NET regulation.METHODS:
NET and NE expression levels were examined by western blot and enzyme-linked immunosorbent assay, respectively. Trophoblast invasion activity, depending on NE-NET regulation, was determined by NET-small interfering RNA (siRNA) and NET transfection into the human extravillous trophoblast cells with or without NE treatment and invasion rates were analyzed by zymography and an invasion assay.RESULTS:
NET mRNA was expressed at a low level in pre-eclamptic placentas compared with normal placentas and NE concentration in maternal plasma increased significantly in pre-eclamptic women compared to normal pregnant women (p<0.05). NET gene upregulation and NE treatment stimulated trophoblast cell invasion up to 2.5-fold (p<0.05) by stimulating matrix metalloproteinase-9 activity via the phosphoinositol-3-kinase/AKT signaling pathway, whereas NET-siRNA with NE treatment reduced invasion rates.CONCLUSION:
NET expression is reduced by inadequate regulation of NE levels during placental development. This suggests that a complementary balance between NET and NE regulates trophoblast cell invasion activities during placental development.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Placenta
/
Placentation
/
Plasma
/
Pre-Eclampsia
/
Trophoblasts
/
RNA
/
RNA, Messenger
/
Enzyme-Linked Immunosorbent Assay
/
Transfection
/
Norepinephrine
Limits:
Female
/
Humans
Language:
English
Journal:
Clinical and Experimental Reproductive Medicine
Year:
2013
Type:
Article
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