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Safety and immunogenicity of recombinant rabies virus (ERAGS) in mice and raccoon dogs
Clinical and Experimental Vaccine Research ; : 159-168, 2016.
Article in English | WPRIM | ID: wpr-176937
ABSTRACT

PURPOSE:

The development of a genetically modified live rabies vaccine applicable to wild raccoon dogs is necessary for the eradication of rabies in Korea. Thus, we constructed a recombinant rabies virus (RABV) called the ERAGS strain, using a reverse genetic system and evaluated its safety and efficacy in mice and its safety and immunogenicity in raccoon dogs. MATERIALS AND

METHODS:

ERAGS, which has Asn194Ser and Arg333Glu substitutions in the glycoprotein, was constructed using site-directed mutagenesis. Mice were inoculated with the ERAGS strain (either 10(5.0) or 10(7.0) FAID(50)/mL) via intramuscular (IM) or intracranial injections and then challenged with a virulent RABV. Raccoon dogs were administered the ERAGS strain (10(8.0) FAID(50)/mL) either orally or via the IM route and the immunogenicity of the strain was evaluated using fluorescent antibody virus neutralization tests.

RESULTS:

The ERAGS strain inoculated into murine neuroblastoma cells reached 10(7.8) FAID(50)/mL at 96-hour post-inoculation. The virus was not pathogenic and induced complete protection from virulent RABV in immunized 4- and 6-week-old mice. Korean raccoon dogs immunized with the ERAGS strain via IM or oral route were also safe from the virus and developed high titer levels (26.4-32.8 IU/mL) of virus-neutralizing antibody (VNA) at 4 weeks post-inoculation.

CONCLUSION:

The ERAGS RABV strain was effectively protective against rabies in mice and produced a high VNA titer in raccoon dogs.
Subject(s)

Full text: Available Index: WPRIM (Western Pacific) Main subject: Rabies / Rabies virus / Raccoons / Neutralization Tests / Vaccines / Rabies Vaccines / Glycoproteins / Mutagenesis, Site-Directed / Raccoon Dogs / Korea Limits: Animals Country/Region as subject: Asia Language: English Journal: Clinical and Experimental Vaccine Research Year: 2016 Type: Article

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Full text: Available Index: WPRIM (Western Pacific) Main subject: Rabies / Rabies virus / Raccoons / Neutralization Tests / Vaccines / Rabies Vaccines / Glycoproteins / Mutagenesis, Site-Directed / Raccoon Dogs / Korea Limits: Animals Country/Region as subject: Asia Language: English Journal: Clinical and Experimental Vaccine Research Year: 2016 Type: Article