An oral Aujeszky's disease vaccine (YS-400) induces neutralizing antibody in pigs
Clinical and Experimental Vaccine Research
;
: 132-137, 2016.
Article
in English
| WPRIM
| ID: wpr-176940
ABSTRACT
PURPOSE:
Aujeszky's disease (AD) is an economically important disease affecting both wild and domestic pigs of the species Sus scrofa. A previous study yielded serological evidence of AD in Korean wild boars, which could spread AD to other animals. A new Aujeszky's disease virus (ADV) bait vaccine is required to prevent AD outbreaks in swine. In the present study, we investigated the safety and immunogenicity of a gE-deleted marker vaccine, strain YS-400, in young domestic pigs. MATERIALS ANDMETHODS:
The YS-400 strain was propagated in Vero cells, and the trial ADV bait vaccine (a vaccine blister in a matrix including an attractant) was prepared. Pigs were orally immunized with the vaccine (2 mL, 10(7.5) TCID(50)/mL) delivered using a syringe or in the bait vaccine. The animals were observed for 9 weeks after vaccination, and immunogenicity was assessed using a virus neutralization (VN) test and enzyme linked immunosorbent assay.RESULTS:
The YS-400 strain was non-pathogenic to pigs when given orally and induced high VN titers (132-1128) 6 weeks post-administration. Of the pigs given the ADV bait vaccine twice or three times, 40% were seropositive by 2 weeks, and 100% were seropositive by 7 weeks after the first dose. Pigs that consumed the AD bait vaccine three times developed VN titers that were slightly higher than those of pigs given the vaccine twice.CONCLUSION:
Domestic pigs given the trial ADV bait vaccine exhibited no adverse effects and developed high VN titers against ADV, indicating that the YS-400 strain is safe and can prevent ADV infection in domestic pigs.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Pseudorabies
/
Swine
/
Syringes
/
Vero Cells
/
Enzyme-Linked Immunosorbent Assay
/
Disease Outbreaks
/
Blister
/
Vaccination
/
Herpesvirus 1, Suid
/
Sus scrofa
Limits:
Animals
Language:
English
Journal:
Clinical and Experimental Vaccine Research
Year:
2016
Type:
Article
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