Efficacy of Dendritic Cells Matured Early with OK-432 (Picibanil(R)), Prostaglandin E2, and Interferon-alpha as a Vaccine for a Hormone Refractory Prostate Cancer Cell Line
Journal of Korean Medical Science
;
: 1284-1290, 2010.
Article
in English
| WPRIM
| ID: wpr-177041
ABSTRACT
Dendritic cells (DCs) are potent antigen-presenting cells. OK432 (Picibanil(R)) was introduced as a potent stimulator of DC maturation in combination with prostaglandin-E2 and interferon-alpha. We compared the efficacy of a DC-prostate cancer vaccine using early-mature DCs stimulated with OK432, PGE2 and INF-alpha (OPA) with that of vaccines using other methods. On days 3 or 7 of DC culture, TNF-alpha (T), TNF-alpha and LPS (TL) or OPA were employed as maturation stimulators. DU145 cells subjected to heat stress were hybridized with mature DCs using polyethyleneglycol. T cells were sensitized by the hybrids, and their proliferative and cytokine secretion activities and cytotoxicity were measured. The yields of early-mature DCs were higher, compared to yields at the conventional maturation time (P<0.05). In the early maturation setting, the mean fusion ratios, calculated from the fraction of dual-positive cells, were 13.3%, 18.6%, and 39.9%, respectively (P=0.051) in the T only, TL, and OPA-treated groups. The function of cytotoxic T cells, which were sensitized with the hybrids containing DCs matured early with OPA, was superior to that using other methods. The antitumor effects of DC-DU145 hybrids generated with DCs subjected to early maturation with the OPA may be superior to that of the hybrids using conventional maturation methods.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Phenotype
/
Picibanil
/
Prostatic Neoplasms
/
Dendritic Cells
/
T-Lymphocytes, Cytotoxic
/
Dinoprostone
/
Lipopolysaccharides
/
Interferon-alpha
/
Cancer Vaccines
/
Cell Line, Tumor
Limits:
Humans
/
Male
Language:
English
Journal:
Journal of Korean Medical Science
Year:
2010
Type:
Article
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