Immunization effect of recombinant P27/30 protein expressed in Escherichia coli against the hard tick Haemaphysalis longicornis (Acari: Ixodidae) in rabbits
The Korean Journal of Parasitology
;
: 195-200, 2004.
Article
in English
| WPRIM
| ID: wpr-177058
ABSTRACT
We investigated the induction of resistance to Haemaphysalis longicornis infestation in rabbits that had been immunized with recombinant H. longicornis P27/30 protein. The success of immunological control methods is dependent upon the use of potential key antigens as tick vaccine candidates. Previously, we cloned a gene encoding 27 kDa and 30 kDa proteins (P27/30) of H. longicornis, and identified P27/30 as a troponin I-like protein. In this study, rabbits that were immunized with recombinant P27/30 expressed in Escherichia coli showed the statistically significant longer feeding duration for larval and adult ticks (P< 0.05), low engorgement rates in larval ticks (64.4%), and an apparent reduction in egg weights, which suggest that H. longicornis P27/30 protein is a potential candidate antigen for a tick vaccine. These results demonstrated that the recombinant P27/30 protein might be a useful vaccine candidate antigen for biological control of H. longicornis.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Tick Infestations
/
Recombinant Proteins
/
Gene Expression
/
Insect Proteins
/
Ixodidae
/
Escherichia coli
/
Microfilament Proteins
/
Antibodies
Limits:
Animals
Language:
English
Journal:
The Korean Journal of Parasitology
Year:
2004
Type:
Article
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