All-Trans Retinoic Acid Has a Potential Therapeutic Role for Diabetic Nephropathy
Yonsei Medical Journal
;
: 1597-1603, 2015.
Article
in English
| WPRIM
| ID: wpr-177064
ABSTRACT
PURPOSE:
The aim of this study was to examine the effects of all-trans retinoic acid (ATRA) on diabetic nephropathy. MATERIALS ANDMETHODS:
We measured amounts of urinary albumin excretion (UAE) after administrating ATRA to Otsuka Long-Evans Tokushima Fatty (OLETF) rats. In order to understand the mechanism of action for ATRA, we administrated ATRA to examine its inhibitory action on the production of transforming growth factor-beta1 (TGF-beta1), protein kinase C (PKC), and reactive oxidative stress (ROS) in cultured rat mesangial cells (RMCs).RESULTS:
After 16 weeks of treatment, UAE was lower in the ATRA-treated OLETF rats than in the non-treated OLETF rats (0.07+/-0.03 mg/mgCr vs. 0.17+/-0.15 mg/mgCr, p<0.01). After incubation of RMCs in media containing 30 or 5 mM of glucose, treatment with ATRA showed time- and dose-dependent decreases in TGF-beta1 levels and ROS. Moreover, ATRA treatment showed a dose-dependent decrease in PKC expression.CONCLUSION:
ATRA treatment suppressed UAE and TGF-beta1 synthesis, which was mediated by significant reductions in PKC activity and ROS production. Our results suggest that ATRA has a potential therapeutic role for diabetic nephropathy.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Tretinoin
/
Reactive Oxygen Species
/
Oxidative Stress
/
Rats, Inbred OLETF
/
Diabetes Mellitus, Type 2
/
Diabetic Nephropathies
/
Mesangial Cells
/
Transforming Growth Factor beta1
Limits:
Animals
Language:
English
Journal:
Yonsei Medical Journal
Year:
2015
Type:
Article
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