Measles Viral Infection in PD-1 Gene Knockout Mice / 소아감염
Korean Journal of Pediatric Infectious Diseases
;
: 123-130, 2013.
Article
in Korean
| WPRIM
| ID: wpr-177243
ABSTRACT
PURPOSE:
Subacute sclerosing panencephalitis (SSPE) is a neurodegerative disease due to persistent measles virus infection. We investigated the role of programmed death-1 (PD-1) molecule which is related with chronic viral infection in developing SSPE in mouse.METHODS:
We adopt the PD-1-/-, PD-1-/+, and wild type BALB/c 3 week old mice to make an animal model of SSPE by injecting measles virus (SSPE strain) intraventricularly. Three months after infusion of virus, the mice were sacrificed and examined if the typical pathologic lesions had been progressed. The sera were collected from each group of mice and the serum level of IL-21 was measured with ELISA kit.RESULTS:
The necrotic lesions on white matter and gliosis were found in focal areas in wild type BALB/c. The extent of lesion was smaller in heterotype BALB/c. Scanty lesions were found in PD-1-/- mice. The sera level of IL-21 was not elevated in all three groups.CONCLUSION:
Our data suggest that the PD-1 molecule may play a role in persistent viral infection.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Subacute Sclerosing Panencephalitis
/
Viruses
/
Enzyme-Linked Immunosorbent Assay
/
Models, Animal
/
Gene Knockout Techniques
/
Gliosis
/
Measles
/
Measles virus
Limits:
Animals
Language:
Korean
Journal:
Korean Journal of Pediatric Infectious Diseases
Year:
2013
Type:
Article
Similar
MEDLINE
...
LILACS
LIS