Effect of Zoledronate on the Expression of Vascular Endothelial Growth Factor-A by Articular Chondrocytes and Synovial Cells: An in Vitro Study
Journal of Bone Metabolism
;
: 249-255, 2014.
Article
in English
| WPRIM
| ID: wpr-177279
ABSTRACT
BACKGROUND:
The aim of this in vitro study was to determine the effect of zoledronate, which is frequently used to treat osteoporosis, on osteoarthritis by analyzing zoledronate-induced expression of vascular endothelial growth factor-A (VEGF-A) in chondrocytes and synovial cells.METHODS:
After chondrocytes and synovial cells were separated and cultured, zoledronate was added, and VEGF-A and pigment epithelium-derived factor (PEDF) expression were quantified by real-time polymerase chain reaction and Western blotting.RESULTS:
There was no significant difference in the expression of VEGF-A mRNA in chondrocytes between the zoledronate group and the control group on the 8th day of culture. The expression of both VEGF-A and PEDF mRNA in synovial cells was significantly decreased in the zoledronate group (P<0.05).CONCLUSIONS:
Zoledronate decreases the expression of VEGF-A in synovial cells and may affect the development and progression of osteoarthritis.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Osteoarthritis
/
Osteoporosis
/
RNA, Messenger
/
Blotting, Western
/
Chondrocytes
/
Vascular Endothelial Growth Factor A
/
Real-Time Polymerase Chain Reaction
Language:
English
Journal:
Journal of Bone Metabolism
Year:
2014
Type:
Article
Similar
MEDLINE
...
LILACS
LIS