The Differential Effects of Donor-Strain Immature Dendritic Cell Subtypes and the Blockade of CD40/CD154 Pathway on the Creation of Allograft Tolerance / 대한신장학회잡지
Korean Journal of Nephrology
;
: 195-204, 2006.
Article
in Korean
| WPRIM
| ID: wpr-17738
ABSTRACT
BACKGOUND Dendritic cells (DC) are pivotal antigen presenting cells and serve a unique role in initiating immunity. To test the hypothesis that pre-immunization of recipient with certain DC subsets of donor origin can influence graft outcome. METHODS:
We have studied the effects of immunization with allogeneic CD4+CD11c+(MDC) and CD8+CD11c+(LDC) DCs on the allograft response.RESULTS:
Both immature MDC and LDC subsets from DBA/2 were able to prime naive allogeneic C57BL/6 (B6) T cells in MLR. In vitro allogeneic T cell responses were attenuated by the addition of anti-CD154 mAb into the culture. T cells from B6 mice that received DBA/2 MDC intravenously 4 weeks before testing mounted weaker MLR driven cell proliferation than T cells from LDC pretreated B6 mice. Consistent with the MLR results, combined pretreatment with MDC, but not LDC, plus anti-CD154 mAb produced donor-strain specific long-term graft survival and induced tolerance while treatment with LDC plus anti-CD154 mAb created minimal prolongation of allograft survival in a pancreas islet transplant model (DBA/2->B6). The beneficial effects exerted by MDC and anti-CD154 mAb pretreatment were correlated with TH1 to TH2 immune deviation and with the amplified donor-specific suppressive capacity by recipient CD4+CD25+T cells.CONCLUSION:
These findings highlight the capacity of MDC to modulate alloimmune responses, and suggest therapeutic approaches for the induction of donor specific tolerance.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Pancreas
/
Tissue Donors
/
Dendritic Cells
/
T-Lymphocytes
/
Immunization
/
Transplants
/
Transplantation Tolerance
/
Cell Proliferation
/
Allografts
/
Graft Survival
Type of study:
Prognostic study
Limits:
Animals
/
Humans
Language:
Korean
Journal:
Korean Journal of Nephrology
Year:
2006
Type:
Article
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