Cause of Intracellular ATP dependency on Zn2++ Blockade of KATP Channels in Pancreatic Beta Cells
Korean Journal of Aerospace and Environmental Medicine
;
: 77-82, 1999.
Article
in Korean
| WPRIM
| ID: wpr-177747
ABSTRACT
In order to clarify the cause of intracellular ATP dependency on Zn2+ blockade of KATP channels in pancreatic beta cells, we investigated the KATP channel activity during external Zn2+ application using voltage clamp technique. Cultured beta cells were used for patch-clamp experiment. When 3 mM glucose was applied in bath, KATP channel activity was increased transiently by externally applied Zn2+ in the cell-attached mode and was recoverable. The KATP channel activity was, however, consistently increased by Zn2+ application during the 0 mM glucose in bath. Inside-out mode, internally applied Zn2+ elicited no response on the KATP channels. Another divalent cation, Mn2+, didn't have any effect on the KATP channels. Therefore, This effect, so-called external glucose-dependency on Zn2+ blockade of the KATP channels, might be due to intracellular Zn2+ metabolism which induces ATP consumption. This appears to be a mechanism that the Zn2+ blockade of the KATP channels in the pancreatic beta cells depends on the intracellular ATP concentration.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Baths
/
Adenosine Triphosphate
/
Insulin-Secreting Cells
/
KATP Channels
/
Glucose
/
Metabolism
Language:
Korean
Journal:
Korean Journal of Aerospace and Environmental Medicine
Year:
1999
Type:
Article
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