Expression of Cyclin D1 and Bcl-xL in Basal Cell Carcinoma and Squamous Cell Carcinoma / 대한피부과학회지

ABSTRACT

BACKGROUND: Cyclin D1 has a key function in cell proliferation. Bcl-xL has anti-apoptotic effects that can protect against cell death. However, it is still largely unknown whether these proteins play important roles in development of basal cell carcinoma (BCC) and squamous cell carcinoma (SCC). OBJECTIVE: The purpose of this study was to investigate the expression of cyclin D1 and Bcl-xL in both of these carcinomas. METHODS: In this study, we examined expression levels of cyclin D1 and Bcl-xL in both carcinomas by immunohistochemistry, immunofluorescence and Western blot analysis. RESULTS: We found increased expression of both proteins in BCC and SCC using immunohistochemistry. Moreover, expression of cyclin D1 and Bcl-xL were increased in both carcinomas relative to normal skin or seborrheic keratosis in Western blot analysis. BCC showed greater increases in expression of cyclin D1 and Bcl-xL on peripheral lesions compared to the center of tumor nests. CONCLUSION: That expression of cyclin D1 and Bcl-xL were increased in BCC and SCC, suggests that progression of the cell cycle combined with inhibition of apoptosis contributes to the carcinogenesis of BCC and SCC.