Development of a Gene Therapy Method for Cervical Cancer Using Attenuated Coxsackievirus B3 as a Vector System
Journal of Bacteriology and Virology
;
: 123-130, 2011.
Article
in Korean
| WPRIM
| ID: wpr-177894
ABSTRACT
We previously reported the development of an attenuated coxsackievirus B3, known as YYFF, which functioned as a viral vector system for foreign gene expression. In this study, we demonstrated the potential use of YYFF as a gene therapy vector. Recombinant YYFF was constructed to express the human papillomavirus 16 (HPV16) E7 gene, referred to as YYFF-HPV16-E7. Growth of YYFF-HPV16-E7 resembled the wild type, YYFF, and it expressed HPV16-E7 in cell culture. When YYFF-HPV16-E7 was directly injected into TC-1-transplanted C57/BL6 mice, there was no reduction in tumor size, because of the non-growth of YYFF in C57/BL6 mice. However, when YYFF-HPV16-E7-induced immune cells/serum that originated from BALB/c mice was passively delivered into BALB/c background TC-1-transplanted nude mice, it reduced the size of cervical tumors in the nude mice. This study indicates the potential use of YYFF-HPV16-E7 as a gene therapy agent for treating HPV-induced cervical cancer.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Genetic Therapy
/
Gene Expression
/
Uterine Cervical Neoplasms
/
Genes, vif
/
Cell Culture Techniques
/
Human papillomavirus 16
/
Mice, Nude
Limits:
Animals
Language:
Korean
Journal:
Journal of Bacteriology and Virology
Year:
2011
Type:
Article
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