Regulation of betaig-h3 Production in Rheumatoid Synovitis by Inflammatory Mediators / 대한류마티스학회지
The Journal of the Korean Rheumatism Association
;
: 73-82, 2005.
Article
in Korean
| WPRIM
| ID: wpr-178152
ABSTRACT
OBJECTIVE:
To investigate the expression pattern of transforming growth factor-beta-inducible gene-h3 (betaig-h3) within rheumatoid synovial tissue and the regulation of betaig-h3 synthesis in fibroblast-like synoviocyte (FLS).METHODS:
Synovial tissues obtained from patients with rheumatoid arthritis and osteoarthritis were obtained during joint replacement surgery. betaig-h3 expression was evaluated with immunohistochemical stain. FLS was isolated from synovial tissues and stimulated with cytokines including TGF-beta, TNF-alpha, IL-1beta, IFN-gamma, IL-6, IL-4, and IL-10. betaig-h3 synthesis was measured using semiquantitative RT-PCR, ELISA, immunofluorescence stain, and flow cytometry.RESULTS:
Expression of betaig-h3 was diffuse and abundant in both lining and sublining layers of rheumatoid synovium, which was more prominent than those of osteoarthritis. Production of betaig-h3 in FLS was regulated by TGF-beta1 in a dose-dependent manner and was highest at 5 ng/mL of TGF-beta1. TNF-alpha and IL-1beta upregulated the production of betaig-h3 from FLS synergistically with TGF-beta1 but other cytokines such as IL-4, IL-6, IL-10 did not affect. betaig-h3 synthesis was efficiently inhibited by dexamethasone at higher dose (100 nM) but not by cyclosporine-A.CONCLUSION:
Production of betaig-h3, which is highly upregulated in rheumatoid synovitis, is differentially regulated by inflammatory cytokines.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Osteoarthritis
/
Arthritis, Rheumatoid
/
Synovial Membrane
/
Synovitis
/
Dexamethasone
/
Enzyme-Linked Immunosorbent Assay
/
Cytokines
/
Transforming Growth Factor beta
/
Fluorescent Antibody Technique
/
Interleukin-4
Limits:
Humans
Language:
Korean
Journal:
The Journal of the Korean Rheumatism Association
Year:
2005
Type:
Article
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