Evaluation of PCR-Reverse Blot Hybridization Assay, REBA Sepsis-ID Test, for Simultaneous Identification of Bacterial Pathogens and mecA and van Genes from Blood Culture Bottles
Annals of Laboratory Medicine
; : 446-455, 2014.
Article
in En
| WPRIM
| ID: wpr-178236
Responsible library:
WPRO
ABSTRACT
BACKGROUND: The aim of this study was to evaluate a newly developed PCR-based reverse blot hybridization assay (PCR-REBA), REBA Sepsis-ID (M&D, Wonju, Korea), to rapidly detect the presence of bacteremia and antimicrobial resistance gene in blood culture samples. METHODS: One thousand four hundred consecutive blood culture samples from patients with a delta neutrophil index greater than 2.7% were selected from March to July in 2013. Three hundred positive and 1,100 negative for bacterial growth in blood culture bottles samples were tested by conventional and real-time PCR-REBA, respectively. RESULTS: The overall agreement between the conventional identification test and the REBA Sepsis-ID test was 95.3% (286/300). Agreement for gram-positive bacteria, gram-negative bacteria, fungi, and polymicrobials was 94.5% (190/201), 97.3% (71/73), 100% (14/14), and 91.7% (11/12), respectively. The detection rate of the mecA gene from methicillin-resistant Staphylococcus isolates was 97.8% (90/92). The vanA gene was detected in one blood culture sample from which vancomycin-resistant Enterococcus was isolated. When the cycle threshold for real-time PCR was defined as 30.0, 2.4% (26/1,100) of negative blood culture samples tested positive by real-time PCR. CONCLUSIONS: The REBA Sepsis-ID test is capable of simultaneously and quickly detecting both causative agents and antimicrobial resistance genes, such as mecA and van, in blood culture positive samples.
Key words
Full text:
1
Index:
WPRIM
Main subject:
Reagent Kits, Diagnostic
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Bacterial Proteins
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RNA, Ribosomal, 16S
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Bacteriological Techniques
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Enterococcus
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Bacteremia
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Carbon-Oxygen Ligases
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Drug Resistance, Bacterial
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Methicillin-Resistant Staphylococcus aureus
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Real-Time Polymerase Chain Reaction
Type of study:
Diagnostic_studies
Limits:
Humans
Language:
En
Journal:
Annals of Laboratory Medicine
Year:
2014
Type:
Article