The Combination of Real-Time PCR and HPLC for the Identification of Non-Tuberculous Mycobacteria
Annals of Laboratory Medicine
;
: 349-352, 2013.
Article
in English
| WPRIM
| ID: wpr-178345
ABSTRACT
We used HPLC and AdvanSure real-time PCR (LG Life Sciences, Korea) to retrospectively analyze non-tuberculous mycobacteria (NTM) in 133 clinical specimens. The specimens were culture-positive for NTM and the HPLC method identified 130 strains of mycobacteria from the cultures (97.7%) at the species level. Among the isolates, 48 Mycobacterium. kansasii (36.1%), 39 M. intracellulare (29.3%), 17 M. avium (12.8%), 16 M. abscessus (12.0%), 6 M. fortuitum (4.5%), 2 M. szulgai (1.5%), 2 M. gordonae (1.5%), and 3 unclassified NTM strains (2.3%) were identified. The real-time PCR assay identified 60 NTM-positive specimens (45.1%), 65 negative specimens (48.9%), and 8 M. tuberculosis (TB)-positive specimens (6.0%). The real-time PCR assay is advantageous because of its rapid identification of NTM. However, in our study, the real-time PCR assay showed relatively low sensitivity (45.1%) when using direct specimens including sputum and bronchoalveolar lavage (BAL) fluid. HPLC is useful as it discriminates NTM at the species level, although it is time-consuming and requires specific equipment and technical expertise. A combination of both methods will be helpful for the rapid and accurate identification of mycobacteria in clinical laboratories.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Sputum
/
DNA, Bacterial
/
Bronchoalveolar Lavage Fluid
/
Chromatography, High Pressure Liquid
/
Real-Time Polymerase Chain Reaction
/
Mycobacterium
/
Mycobacterium Infections
Type of study:
Diagnostic study
/
Prognostic study
Limits:
Aged
/
Aged80
/
Female
/
Humans
/
Male
Language:
English
Journal:
Annals of Laboratory Medicine
Year:
2013
Type:
Article
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