Effects of Polycaprolactone-Tricalcium Phosphate, Recombinant Human Bone Morphogenetic Protein-2 and Dog Mesenchymal Stem Cells on Bone Formation: Pilot Study in Dogs
Yonsei Medical Journal
;
: 825-831, 2009.
Article
in English
| WPRIM
| ID: wpr-178453
ABSTRACT
PURPOSE:
The aim of this study was to evaluate the survival, proliferation, and bone formation of dog mesenchymal stem cells (dMSCs) in the graft material by using Polycaprolactone-tricalcium phosphate (PCL-TCP), auto-fibrin glue (AFG), recombinant human bone morphogenetic protein-2 (rhBMP-2), and dMSCs after a transplantation to the scapula of adult beagle dogs. MATERIALS ANDMETHODS:
The subjects were two beagle dogs. Total dose of rhBMP-2 on each block was 10 microg with 50 microg/mg concentration. The cortical bone of the scapula of the dog was removed which was the same size of PCL-TCP block (Osteopore International Pte, Singapore; 5.0x5.0x8.0 mm in size), and the following graft material then was fixed with orthodontic mini-implant, Dual-top(R) (Titanium alloy, Jeil Co. Seoul, Korea). Four experimental groups were prepared for this study, Group 1 PCL-TCP + aFG; Group 2 PCL-TCP + aFG + dMSCs; Group 3 PCL-TCP + aFG + dMSCs + rhBMP-2; Group 4 PCL-TCP + aFG + dMSCs + rhBMP-2 + PCL membrane. The survival or proliferation of dMSCs cells was identified with an extracted tissue through a fluorescence microscope, H-E staining and Von-Kossa staining in two weeks and four weeks after the transplantation.RESULTS:
The survival and proliferation of dMSCs were identified through a fluorescence microscope from both Group 1 and Group 2 in two weeks and four weeks after the transplantation. Histological observation also found that the injected cells were proliferating well in the G2, G3, and G4 scaffolds.CONCLUSION:
This study concluded that bone ingrowth occurred in PCL-TCP scaffold which was transplanted with rhBMP-2, and MSCs did not affect bone growth. More sufficient healing time would be needed to recognize effects of dMSCs on bone formation.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Osteogenesis
/
Polyesters
/
Recombinant Proteins
/
Calcium Phosphates
/
Fibrin Tissue Adhesive
/
Cell Survival
/
Cells, Cultured
/
Transforming Growth Factor beta
/
Bone Morphogenetic Proteins
/
Mesenchymal Stem Cell Transplantation
Type of study:
Prognostic study
Limits:
Animals
/
Humans
Language:
English
Journal:
Yonsei Medical Journal
Year:
2009
Type:
Article
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