Biological Activities and Stability of a Standardized Pentacyclic Triterpene Enriched Centella asiatica Extract
Natural Product Sciences
;
: 20-24, 2016.
Article
in English
| WPRIM
| ID: wpr-178615
ABSTRACT
Pentacyclic triterpenes, mainly, asiatic acid, madecassic acid, asiaticoside, and madecassoside are the active constituents of Centella asiatica. A pentacyclic triterpene enriched C. asiatica extract (PRE) was prepared and standardized to contain a total pentacyclic triterpenes not less than 65% w/w. This work was focused on determination of antiinflammatory, antioxidant, and tyrosinase inhibitory activities of PRE and its stability. The PRE exhibited a satisfactory nitric oxide inhibitory effect, with an IC50 value of 64.6 µg/mL. In addition, the PRE inhibited tyrosinase enzyme activity with an IC50 value of 104.8 µg/mL. In contrast, the PRE possessed only weak antioxidant activity. The PRE was stable over a period of four months when stored as a dried powder but only in a well-closed container protected from light at 4 °C. An aqueous alcoholic solution of the PRE was stable at pH values of 5.8 and 7.0, but was not stable at a pH of 8.2. Preparations of the PRE in an aqueous solution should be performed in acidic or neutral conditions.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Monophenol Monooxygenase
/
Inhibitory Concentration 50
/
Centella
/
Pentacyclic Triterpenes
/
Alcoholics
/
Hydrogen-Ion Concentration
/
Nitric Oxide
Limits:
Humans
Language:
English
Journal:
Natural Product Sciences
Year:
2016
Type:
Article
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