Extracellular K+ Effects on the Mouse Aortic Endothelial Cell Contractility / 대한흉부외과학회지
The Korean Journal of Thoracic and Cardiovascular Surgery
;
: 887-893, 2003.
Article
in Korean
| WPRIM
| ID: wpr-179023
ABSTRACT
BACKGROUND:
External stimuli increases intracellular (IC) Ca2+, which increases extracellular (EC) K+. To verify K+ effects on the vascular contraction, we performed an experiment using mouse aortic endothelial cell. MATERIAL ANDMETHOD:
We examined the mouse aortic contractility changes as we measured the IC Ca2+ change and ionic current by using the voltage clamp technique under different conditions such as; increasing EC K+, removing endothelial cell, giving L-NAME (N-nitro-L-arginine methyl ester) which suppress nitric oxide formation, Ouabain which control Na+-K+ pump and Ni2+ which repress Na+-Ca2+ exchanger.RESULT:
When we increased EC K+ from 6 to 12 mM, there was no change in aortic contractility. Aorta contracted with more than 12 mM of EC K+. Acetylcholine (ACh) induced relaxation was inhibited with EC K+ from 6 to 12 mM, but was not found after de- endothelialization or L-NAME treatment. ATP or ACh increased IC Ca2+ in cultured endothelium. After maximal increase of IC Ca2+, increasing EC K+ from 6 to 12 mM made IC Ca2+ decrease and re-decreasing EC K+ to 6 mM made IC Ca2+ increase. Ouabain and Ni2+ masked the inhibitory effect of endothelium dependent relaxation by increased EC K+.CONCLUSION:
These data indicate that increase in EC K+ relaxes vascular smooth muscle and reduces Ca2+ in the endothelial cells which inhibit endothelium dependent relaxation. This inhibitory mechanism may be due to the activation of Na+-K+ pump and Na+-Ca2+ exchanger.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Aorta
/
Ouabain
/
Potassium
/
Relaxation
/
Acetylcholine
/
Adenosine Triphosphate
/
NG-Nitroarginine Methyl Ester
/
Endothelial Cells
/
Endothelium-Dependent Relaxing Factors
/
Endothelium
Limits:
Animals
Language:
Korean
Journal:
The Korean Journal of Thoracic and Cardiovascular Surgery
Year:
2003
Type:
Article
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