Retroviral-mediated IL-12 gene therapy for advanced murine tumors
Experimental & Molecular Medicine
;
: 53-58, 1997.
Article
in English
| WPRIM
| ID: wpr-179544
ABSTRACT
Interleukin 12 (IL-12), a heterodimeric cytokine, promotes an effective antitumor response against tumors of various histological types when delivered systemically as a protein or locally by gene transfer. We investigated parameters that influenced the effectiveness of IL-12 retroviral-mediated gene therapy of cancer in animals using the murine breast cancer line TS/A. Syngeneic fibroblasts (TIB80), stably transduced with a retrovirus expressing murine IL-12, were used for peritumoral injection. Injection of fibroblasts into established tumors resulted in complete regression of tumor in 40 % of animals in a dose dependent manner when treated on day 4, and 20 % when treated on day 8. Significant inhibition of growth of day 21 and day 40 tumors was observed following peritumoral injection of IL-12-expressing fibroblasts in a dose-dependent manner. Delivery of IL-12 by syngeneic fibroblasts at a tumor site is effective in eradicating established, weakly immunogenic TS/A tumors.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Retroviridae
/
Breast Neoplasms
/
Genetic Therapy
/
Interleukin-12
/
Fibroblasts
Limits:
Animals
Language:
English
Journal:
Experimental & Molecular Medicine
Year:
1997
Type:
Article
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