Comparison of Sensitivity of Tests for Detecting Bacterial Contamination in Platelet Concentrates / 대한수혈학회지
Korean Journal of Blood Transfusion
;
: 32-39, 2009.
Article
in Korean
| WPRIM
| ID: wpr-179782
ABSTRACT
BACKGROUND:
The demand for platelet concentrates has increased for patients with hemato-oncologic diseases as well as for patients with chronic diseases. As platelet concentrates are preserved at 22~24degrees C, the chance of bacterial contamination exposure is increased, which can cause fatal outcomes. We evaluated various methods for detecting bacterial contamination in platelet concentrates.METHODS:
0.5 MacFarland standard solutions were prepared using the Staphylococcus aureus ATCC 25923 & Escherichia coli ATCC25922 strains. The platelet concentrates were inoculated with various concentrations (10(1)~10(5) CFU/mL) of bacteria and then gram staining, plate culture, broth culture and 16s RNA were used to detect bacteria.RESULTS:
The gram stain method was unable to detect bacteria concentrations less than 10(4) CFU/mL. The plate culture method detected bacterial growth concentrations up to 10(3) CFU/mL, but only 1 specimen of S. aureus was detected at the lowest concentration of 10(1) CFU/mL. The broth culture method detected 10(2) CFU/mL concentrations except for samples from S. aureus and E. coli strains. Among the 10(1) CFU/mL lowest concentrations, bacterial growth detected 3 samples from S. aureus and 2 samples from E. coli. For the broth culture method, detection of bacterial growth up to 10(1) CFU/mL took 58.9 hours, it took 57.5 hours for S. aureus and E. coli respectively, and it took 43.9 hours and 49.0 hours for 10(2) CFU/mL concentrations of S. aureus and E. coli, respectively. The PCR method showed all positive results except for 1 specimen of E. coli.CONCLUSION:
The broth culture method showed similar sensitivity to PCR except for the 43.9~58.9 hours of an incubation period to show positive RESULTS. Overall, the PCR method was most sensitive and rapid method for detecting bacterial contamination in platelet concentrates.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Staphylococcus aureus
/
Bacteria
/
Blood Platelets
/
RNA
/
Polymerase Chain Reaction
/
Chronic Disease
/
Fatal Outcome
/
Escherichia coli
Type of study:
Diagnostic study
Limits:
Humans
Language:
Korean
Journal:
Korean Journal of Blood Transfusion
Year:
2009
Type:
Article
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