Effect of Trypan Blue on the Survival of Cultured Trabecular Meshwork Cells
Journal of the Korean Ophthalmological Society
;
: 327-332, 2017.
Article
in Korean
| WPRIM
| ID: wpr-179982
ABSTRACT
PURPOSE:
To evaluate the effects of trypan blue (TB) on the survival of cultured human trabecular meshwork cells (HTMCs).METHODS:
Primarily cultured HTMCs were exposed to 0.05, 0.10 or 0.50% TB for 1, 5 or 30 min. Cellular survival was assessed using the MTT assay and degree of apoptosis was analyzed with flow cytometry using annexin-V/propidium iodide double staining.RESULTS:
Long-term exposure or high concentration of TB decreased the survival of HTMCs (p < 0.05). In flow cytometric analysis, exposure to 0.50% TB for 30 min increased the degree of apoptosis (p < 0.05). Commercial TB decreased cell survival after exposure for 5 min and increased the degree of apoptosis after exposure for 30 min (p < 0.05).CONCLUSIONS:
TB may cause cellular damage of cultured HTMCs and apoptosis could be the underlying mechanism. In TB-assisted cataract surgery, TB should be used for the shortest time possible and removed completely.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Trabecular Meshwork
/
Trypan Blue
/
Cataract
/
Cell Survival
/
Apoptosis
/
Flow Cytometry
Limits:
Humans
Language:
Korean
Journal:
Journal of the Korean Ophthalmological Society
Year:
2017
Type:
Article
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