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Microarray Analysis for Genes Associated with Angiogenesis in Diabetic OLETF Keratocytes
Journal of Korean Medical Science ; : 265-271, 2014.
Article in English | WPRIM | ID: wpr-180432
ABSTRACT
The purpose of this study was to identify the differences in angiogenesis gene expression between normal and diabetic keratocytes stimulated with interleukin-1alpha (IL-1alpha) and tumor necrosis factor-alpha (TNF-alpha). Primarily cultured normal and diabetic keratocytes were treated with 20 ng/mL of IL-1a and TNF-alpha for 6 hr. cDNA was hybridized to an oligonucleotide microarray. Microarray analysis was used to identify differentially expressed genes that were further evaluated by real-time polymerase chain reaction (RT-PCR). Diabetes keratocytes overexpressed vital components of angiogenesis including Agtr1, and under-expressed components related to the blood vessel maturation, including Dcn. Cytokine-treated diabetic keratocytes differentially expressed components of angiogenesis. OLETF keratocytes after treatment with IL-1alpha and TNF-alpha showed the newly expressed 15 and 14 genes, respectively. Newly and commonly under-expressed five genes followed by treatment with both IL-1alpha and TNF-alpha were also evident. RT-PCR showed results similar to the microarray results. Agtr1 and Itga1 showed an increased expression in diabetic keratocytes compared with normal corneal keratocytes, especially after TNF-alpha treatment. Il6 appeared strong expression after interleukin-1alpha treatment, but showed down expression after TNF-alpha treatment. Further studies to analyze and confirm the significance of the identified angiogenetic genes of diabetes are needed.
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Full text: Available Index: WPRIM (Western Pacific) Main subject: Keratinocytes / Cells, Cultured / Gene Expression Regulation / Tumor Necrosis Factor-alpha / Neovascularization, Physiologic / Oligonucleotide Array Sequence Analysis / Receptor, Angiotensin, Type 1 / Interleukin-1alpha / Real-Time Polymerase Chain Reaction Type of study: Prognostic study Limits: Animals Language: English Journal: Journal of Korean Medical Science Year: 2014 Type: Article

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Full text: Available Index: WPRIM (Western Pacific) Main subject: Keratinocytes / Cells, Cultured / Gene Expression Regulation / Tumor Necrosis Factor-alpha / Neovascularization, Physiologic / Oligonucleotide Array Sequence Analysis / Receptor, Angiotensin, Type 1 / Interleukin-1alpha / Real-Time Polymerase Chain Reaction Type of study: Prognostic study Limits: Animals Language: English Journal: Journal of Korean Medical Science Year: 2014 Type: Article