The Effect of Intra-portal Infusion of Glucose-insulin-potassium (GIK) Solution on the Energy Metabolism during Cold Preservation in a Small-animal Model for Liver Transplantation / 대한이식학회지
The Journal of the Korean Society for Transplantation
;
: 41-48, 2008.
Article
in Korean
| WPRIM
| ID: wpr-180621
ABSTRACT
PURPOSE:
The effect of intra-portal infusion of glucose-insulin-potassium (GIK) solution on the energy metabolism during cold preservation was investigated using a small-animal liver transplantation model.METHODS:
Fifteen white rats were divided into 3 groups the group A (feeding group) were fed normally before experiment. The group B (fasting group) and group C (GIK group) were fasted from 3 days before experiment, by which acute nutritional deficiency state was induced. In group A and B, the whole liver was procured after intra-portal perfusion of HTK solution and serial liver biopsies were performed during the cold preservation period with 4degrees C HTK solution. In group C, intra-portal GIK solution infusion for 1 hour preceded liver graft harvest. From the liver tissues, the relative intracellular glycogen contents and the ATP concentration were measured.RESULTS:
Relative glycogen contents in group A were 100% at 0 h, 64.6% at 2 h, 54.9% at 4 h, and 16.2% at 8 h; 10.3%, 8.3%, 4.9% and 0%, respectively in group B; 109.2%, 96.9%, 54.2% and 9.7%, respectively in group C. There was a temporary supercharge of ATP level in group C only at 0 h. Apoptosis was less expressed in group C comparing with group A and B.CONCLUSION:
Rapid intra- portal infusion of GIK solution could make intrahepatic glycogen content fully restored to the normal level. Considering that intracellular glycogen is the main energy source during immediate post-transplant period, its restoration may contribute to improvement of post-transplant graft function.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Perfusion
/
Potassium
/
Potassium Chloride
/
Procaine
/
Biopsy
/
Adenosine Triphosphate
/
Liver Transplantation
/
Apoptosis
/
Cold Temperature
/
Transplants
Limits:
Animals
/
Humans
Language:
Korean
Journal:
The Journal of the Korean Society for Transplantation
Year:
2008
Type:
Article
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