The Effect of FK506 to Generate Reactive Oxygen Species on T Lymphocyte Death
Journal of the Korean Surgical Society
;
: 310-319, 2009.
Article
in Korean
| WPRIM
| ID: wpr-181023
ABSTRACT
PURPOSE:
Tacrolimus (FK506) has been widely used as an immunosuppressant in organ transplanted recipients to suppress organ rejection phenomenon. We investigated the role of oxidative stress and heme oxygense-1 by FK506 on human Jurkat T cells.METHODS:
The cells viability was examined by DAPI stain, enzyme activity of caspase family proteins, and western blotting for Baks, PUMA, iNOS, HO-1. Cells were cultured in the absence or presence of CoPPIX or ZnPPIX and the fluorescence intensity was analyzed using a flow cytometry.RESULTS:
Treatment with FK506 increased the generation of reactive oxygen species (ROS), including hydrogen peroxide and superoxide anion, and NO in Jurkat cells in a dose-dependent manner. Immunohistochemistry and Western blot analysis data revealed the hemoxygenase-1 (HO-1) was induced by the addition of FK506 in Jurkat cells. Induction of CoPP, HO-1 inducer, resulted in decreased intracellular H2O2 and NO concentrations. Instead ZnPP, an HO-1 competitive inhibitor did it reversely. In addition, ZnPP regulates iNOS protein synthesis by inhibition of HO-1.CONCLUSION:
Increase of HO-1 expression would induce to decrease the intracellular H2O2 and NO concentrations. Also, HO-1 would regulate iNOS protein synthesis. Consequently, we can expect the regulation of HO-1 expression with concomitants use of FK506 to suppress organ rejection phenomenon by enhancing apoptosis.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Rejection, Psychology
/
Immunohistochemistry
/
Lymphocytes
/
T-Lymphocytes
/
Proteins
/
Blotting, Western
/
Tacrolimus
/
Reactive Oxygen Species
/
Apoptosis
/
Superoxides
Limits:
Humans
Language:
Korean
Journal:
Journal of the Korean Surgical Society
Year:
2009
Type:
Article
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