Change of Chemosensitivity and Cellular Characteristics of Cisplatinum Resistant Human Bladder Cancer Cells Induced by Long-term Cisplatinum Treatment / 대한비뇨기과학회지
Korean Journal of Urology
;
: 305-310, 1998.
Article
in Korean
| WPRIM
| ID: wpr-181531
ABSTRACT
PURPOSE:
Cisplatinum has the therapeutic efficacy against a bladder cancer. However, the response is often limited due to appearance of drug-resistant tumor cells. The studies of establishment and characterization of cisplatinum-resistant tumor cells are considered to be helpful in elucidating the underlying mechanism of acquired resistance to cisplatinum in human tumors. MATERIALS ANDMETHODS:
We established cisplatinum-resistant cell lines sequentially, T24Rl and T24R2, which show resistance to cisplatinum at a concentration of 1 and 2microgram /ml, respectively, by the stepwise exposure of T24 human bladder cancer cell to increasing concentrations of cisplatinum.RESULTS:
The resistance to cisplatinum of T24Rl and T24R2 cells was 13- and 18-fold that of the parental T24 cells, respective1y. Growth, DNA synthesis and cell cycle distribution of T24Rl and T24R2 cells were not different from those of the parental T24 cells.CONCLUSIONS:
These cisplatinum-resistant bladder tumor cell lines could be a useful model to elucidate the biochemical and molecular mechanism Involved In the cisplatinum resistance.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Parents
/
Urinary Bladder
/
Urinary Bladder Neoplasms
/
DNA
/
Cell Cycle
/
Cell Line
Type of study:
Prognostic study
Limits:
Humans
Language:
Korean
Journal:
Korean Journal of Urology
Year:
1998
Type:
Article
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