Effects of Interleukin 4 on the Production of Interleukin 6 in Human Keratinocytes / 대한피부과학회지
Korean Journal of Dermatology
;
: 847-854, 1995.
Article
in Korean
| WPRIM
| ID: wpr-181635
ABSTRACT
BACKGROUND:
The human keratinocyte can synthesize interleukin 6 (IL-6) under certain conditions, and the IL-6 synthesis is inhibited by interleukin 4 (IL-4) in the human monocyte.OBJECTIVE:
To find out what kind of stimulating agents can induce the IL-6 production and whether IL-4 affects the production of IL-6 in the human cultured keratinocytes.METHODS:
We stimulated the keratinocytes with either lipopolysaccharide (LPS), fetal bovine serum (FBS), human recombinant interferon-gamma(IFN-gamma) to induce the IL-6 production, and treated the keratinocytes, which stimulated either with 10% FBS or human recombinant IFN-gamma, with human recombinant IL-4.RESULTS:
The LPS stimulation resulted in no increase of IL-6 levels in the keratinocyte supernatants. When the keratinocytes were stimulated either with 1%, 5%, 10% FBS with or without 5 microgram/ml LPS, significantly increased amounts of IL-6 were detected. The level of IL-6 in the keratinocytes treated with the human recombinant IFN-gamma increased, too. The human recombinant IL-4 downregulates the secretion of IL-6 by the keratinocytes which were activated either with 10% FBS or human recombinant IFN-gamma.CONCLUSION:
We have shown that it is possible to induce the IL-6 synthesis by stimulating the keratinocytes and that human recombinant IL-4 profoundly inhibits the synthesis of IL-6. So we suggest that there may be a cytokine network which regulates the primary immune response in the skin.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Skin
/
Monocytes
/
Keratinocytes
/
Interleukins
/
Interleukin-4
/
Interleukin-6
Limits:
Humans
Language:
Korean
Journal:
Korean Journal of Dermatology
Year:
1995
Type:
Article
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