Quantification of endothelin-1 in human umbilical venous endothelial cell culture supernatants of small for gestational age and preeclampsia neonates / 소아과
Korean Journal of Pediatrics
;
: 1194-1199, 2007.
Article
in Korean
| WPRIM
| ID: wpr-182368
ABSTRACT
PURPOSE:
It was generally accepted now a days that the pathogenesis of preeclampsia, small for gestational age (SGA), intrauterine growth retardation and fetal origin of adult diseases were related with a endothelial cell dysfunction. The purpose of this study was to know the relation of such diseases by assessing the level of endothelin-1.METHODS:
SGA babies, newborns of preeclampsia and normal control mother were included in this study. Isolated endothelial cells were centrifugated and mixed with media in 37, 5% CO2 to obtain confluent monolayer of cultured human umbilical venous endothelial cell (HUVEC). Endothelin-1 levels were determined by Endothelin-1 colorimetric (EIA) Kits. We examined the endothelin-1 level in the HUVEC supernatants from SGA baby, and newborns from preeclampsia as well as normal mother. Also, we compared the endothelin-1 level of cultured normal HUVEC incubated with serum from cord blood of SGA, babies of preeclampsia or normal control mother.RESULTS:
The endothelin-1 levels in cultured HUVEC supernatants of three groups showed no significant difference but the endothelin-1 levels of cultured normal HUVEC incubated with serum from preeclampsia mother or SGA mother was significantly higher than those from newborns of control mothers (P<0.05).CONCLUSION:
These findings suggest that there may be the factor which affect the endothelin-1 level in serum of cord blood from SGA and preeclampsia.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Pre-Eclampsia
/
Umbilical Veins
/
Gestational Age
/
Endothelin-1
/
Endothelial Cells
/
Fetal Blood
/
Fetal Growth Retardation
/
Mothers
Limits:
Adult
/
Humans
/
Infant, Newborn
Language:
Korean
Journal:
Korean Journal of Pediatrics
Year:
2007
Type:
Article
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