Inhibitory Effect of Angiotensin Blockade on Hepatic Fibrosis in Common Bile Duct-ligated Rats / 대한간학회지
The Korean Journal of Hepatology
;
: 61-69, 2007.
Article
in Korean
| WPRIM
| ID: wpr-182808
ABSTRACT
BACKGROUNDS AND AIMS:
Angiotensin receptors are found on hepatic stellate cells, which participate in hepatic fibrosis. Therefore, it is presumed that angiotensin has a role in hepatic fibrosis. The aim of this study was to evaluate the effects of angiotensin blockade on inhibition of hepatic fibrosis in cirrhotic rat model. Material andMETHODS:
Cirrhosis with portal hypertension was produced by common bile duct ligation (BDL) in the adult Sprague-Dawley rats. They were classified into 4 groups (each group n=6) as follows; G1 BDL without drug, G2 BDL+captopril 100 mg/kg/day beginning 2 weeks after BDL, G3 BDL+captopril 100 mg/kg/day, starting just after BDL, G4 BDL+losartan 10 mg/kg/day, starting just after BDL. After 4 weeks following BDL, hepatic fibrosis was histomorphologically analyzed by Batts & Ludwig score. Alpha smooth muscle actin by immunohistochemical stain, hydroxyproline contents of liver tissue by spectrophotometry and expression of collagen, procollagen, and TGF-beta by real-time PCR were measured.RESULTS:
Batts & Ludwig score were 3.8, 3.0, 2.6,and 2.6 in G1, G2, G3, and G4, respectively. The expression of alpha-SMA was significantly lower in G3 and G4 than in G1; 11.9%, 10.9%, 2.6%, and 1.1% in G1, G2, G3, and G4, respectively (p<0.05). The concentration of hydroxyproline (microgram/g liver tissue) was lower in G3 and G4 compared with G1 (p<0.05). Also, the administration of angiotensin blockade just after BDL significantly reduced the expression of collagen, procollagen, and TGF-beta mRNA.CONCLUSIONS:
Angiotensin blockades are effective in the prevention of hepatic fibrosis in BDL rats.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Bile Ducts
/
Fibrosis
/
Captopril
/
Transforming Growth Factor beta
/
Actins
/
Rats, Sprague-Dawley
/
Losartan
/
Angiotensin II Type 1 Receptor Blockers
/
Hydroxyproline
/
Ligation
Type of study:
Prognostic study
Limits:
Animals
Language:
Korean
Journal:
The Korean Journal of Hepatology
Year:
2007
Type:
Article
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