E-cadherin and beta-catenin Expression and Mutation in Gastric Carcinomas
Journal of the Korean Gastric Cancer Association
;
: 202-209, 2001.
Article
in Korean
| WPRIM
| ID: wpr-183102
ABSTRACT
PURPOSE:
When cancer cels invade the stroma, they should be dissociated from the adjacent cells at first. E-cadherin and beta-catenin constitute an important protein complex associated with cellular adhesion, development, and differentiation, especially in epithelial cells.The role of E-cadherin and beta- catenin in gastric carcinogenesis were studied. MATENRIALS ANDMETHODS:
The expression of E-cadherin and beta-catenin in gastric adenocarcinomas by using immunohistochemical staining and the mutation by using polymerase chain reaction- single stranded conformation polymorphism (PCR-SSCP) and sequencing were performed in 40 adenocarcinomas and 5 dysplasia of stomach. Thirteen cases, which had lymph node metastasis, were also included for immunohistochemical staining.RESULTS:
Inappropriate cytoplasmic and/or nuclear expression of a E-cadherin-beta-catenin complex was more frequent in poorly differentiated, diffuse type signet ring cell carcinomas than in well-differentiated, intestinal type adenocarcinomas (P<0.05). However, the expression was not related with clinical stage or lymph node metastasis. Mutation of E-cadherin was detected in 4 cases by using PCR-SSCP, whereas mutation of beta-catenin was detected in 2 cases.CONCLUSION:
E-cadherin and beta-catenin seem to be important in gastric carcinogenesis, especially in poorly differentiated diffuse type.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Stomach
/
Immunohistochemistry
/
Adenocarcinoma
/
Cadherins
/
Carcinoma, Signet Ring Cell
/
Cytoplasm
/
Beta Catenin
/
Carcinogenesis
/
Lymph Nodes
/
Neoplasm Metastasis
Language:
Korean
Journal:
Journal of the Korean Gastric Cancer Association
Year:
2001
Type:
Article
Similar
MEDLINE
...
LILACS
LIS